Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. It is designed to work seamlessly with a variety of 96 well microplate readers, including popular brands such as Agilent™, Berthold™, Biotek™, BMG LABTECH™, Molecular Devices™, Perkin Elmer™, Promega™, Tecan™, and. The reduced cost of the device represents the major technical contribution of this manuscript, as costs were reduced 7 fold from previous reports. Fluorescence Microplate Reader Comparison Chart. The SpectraMax® L microplate reader measures flash and glow luminescence assays in both 96- and 384-well plates. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Three kits covering a wide range of dsDNA concentrations. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. robotics-automation-compatible. 1 fmol luciferase per well. A 96-well microplate reader for absorption spectroscopy was designed, constructed, and tested at a total cost of ca. This instrument is ideal for measuring dual luciferase assays in both 96- and 384-well plates, and SoftMax Pro® software simplifies data collection and analysis with preconfigured protocols. Kinetic assays were read every 20-28 seconds for one hour,The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. $3,207. The color touch screen provides a visual user interface, making programming fast and intuitive. 300 µL of Detergent Compatible Bradford reagent was added to each well. 6- to 1536-well plates 4 96- to 384-well plates 4 With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. • Remove media from each well using a blue pipette tip being careful as not to remove cells. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). 3) At the bottom of the strip, click on "Show. Each wellThe Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. 9. Temperature control (RT+3°C – 42°C) Liquid dispensers with reagent heater and stirrer. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. To help you get the best results from your Molecular Devices® plate readers it is essential that you choose the right plate. Published Dec 16, 2020 | Updated Dec 16, 2020. 190. 2% in. 00. The IC50s by cell rounding and LysoTracker. 000 ABS • Resolution: 0. \r Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. 100 μL of each endotoxin standard was added to triplicate wells of an LAL Reagent Grade Multi-well Plate. Fluorescence Microplate Reader Comparison Chart. 000 – 4. The ImageXpress® Pico Automated Cell Imaging System is more than a digital microscope, combining high-resolution imaging with powerful analysis. x. Related Products: 96 Well Plate Reader. 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started. Suggested plates are black walled. Highly selective for dsDNA over ssDNA or RNA. The entire Bulk Kit is sufficient for 100 plates (96- and 384-well), or 70 plates (1536-well). It also integrates with the powerful SoftMax Pro Software for. #R8195. Sensitive luminometer with programmable injector options for 96- and 384-well microplates. The clear bottom plates offer the advantage of being able to examine the cells by microscopy during the course of the. Microplates, cuvettes (via adapter) Detectors. The onboard luminescence detection mode may be used, or, for higher sensitivity, the reader can be equipped with a NanoBRET detection cartridge that offers a 4-fold improvement in limit of quantitation. With various on-board features and capabilities, the ThermoMax. The system can perform readings. Software Validation. Primary considerations when choosing a microplate reader are throughput and flexibility. Molecular Devices, LLC Plate Formats: 96 or 384 Well Plates; Manifold Channels: Inquire; Dispensing Liquids: Inquire; Citations: Get Quote. Align the two slots on the bottle holder with the two pegs on the rail. Turn on Plate Reader and wait for it to complete its start-up routine. Including injection time, it takes approximately 68 secs. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. SpectraTest FL1 Fluorescence Validation Plate User Guide Molecular DevicesData are shown as the mean ± s. C. 5. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. adjust the reader. Bowman Dispensers. Clear plates will give more cross talk between wells. Our washers provide speed and configurability options—from single-tube dispensing to washing full 96- and 384-well plates. The Varioskan LUX multimode reader has received HTRF™ certification from Cisbio Bioassays and DLReady™ validation from Promega Corporation. Fireflysci UV/VIS Fluorescence Validation Plate for Well Plate Reader (290-562nm) FLVP-01. They can be read by most microplate readers. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). 312 gain calibration failed. In addition, the iD5 reader measures TRF and FP and. Reads various kinds of 96-wells plates, and is equipped with shaking function. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads the VMax Microplate Reader has both speed and performance. For the kinetic and endpoint LAL assays, the ELx808 TM Reader is highly reliable and easy to use. We would like to show you a description here but the site won’t allow us. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. guaranteed 25 fmol/200 µL fluorescein (125 pM), black 96-well plate; 1 second integration time 15 fmol/75 µL. • Easy and gentle immobilization of biotin. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. Incubation to 70 o C enables high temperature assays such as enzyme kinetics, protein stability/aggregation. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. 96-well Molecular Devices P/N 42-000-0117 HE™ High Efficiency microplates, 384-well Molecular Devices P/N 0200-5201. 039. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. Sort By: Show:. Making it possible to perform kinetic analysis of solid. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Performing molecular and cellular analyses in multiwell microplate formats is an important methodology for high-throughput screening, or for boosting throughput generally. Contents of wells were pipetted up and down several times to mix. F3/F5 Multi-Mode Microplate ReadersAnalyst® Multimode Reader—one of the following: • Acquest or Analyst AD or HT with at least v. x. Source plate capacity; 1 x plates. 76358-624. Or scan up to 384 wells at any time to determine the best assay conditions. Service parts for these models are not being produced and are no longer in stock. Microplate Readers include the M3, M4, M5, and M5e models. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. Product Compare 0. minutes and begin reading microplates with seven mouse clicks. This reader's dual monochromators for. The CellTiter-Glo assay was used to measure ATP. 0% ± 0. Supplier: Molecular Devices. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. 9000-0756. xThe SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. The absorbance was then read on the SpectraMax microplate reader at 426 nm, and the resulting OD values for all wells were averaged together to obtain a value of 0. * Note: Programming the washer with a final aspirate height of 4. Quantitative, whole well cell confluence for every well. However, for greater flexibility, some instruments can accommodate 6- to 1536-well plate formats. 003 fmol 0. 0. Polypropylene Deep well Plates are used as small test tubes because of easier sealing, flat or conical shape, square and round well formats with lids and cap. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. #ID5-STD) Pierce BCA Protein Assay Kit (ThermoFisher Scientific cat. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Click “Next”, and the reader will start scanning the four corner wells; a progress indicator will appear: fter the reader finishes scanning the four corner wells, you will see a screen with an image of the upper left corner A well and a pink crosshair. 00 Add to cart; Molecular Devices EMax Microplate Reader $. Dear Nelly, In a micro plate the fluorescence emission optics are very different from those in a single cell because of obstruction, reabsorption etc. Instrument settings can beIntegrating ValitaTiter and Molecular Devices fluorescence polarization (FP) plate readers produces a seamless IgG titer assay setup and optimization. Z. The SpectraMax i3x Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. SpectraMax ID5 Plate Reader (Molecular Devices) Description Internal price (Cornell and Cornell affiliates) External Price; SpectraMax ID5 Plate Reader Use: $20/hr: $32/hr. Temperature variation ± 0. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and. . Published Dec 16, 2020 | Updated Dec 16, 2020. 5 second read per plate. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. Plate types Flat and round 96-well plates Data output Export to PC Detection system Single channel silicon photodiode Reading speed 25 seconds Software SoftMax Pro Software Shaking None Temperature control None Linearity 0. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. 96-, 384-, and 1536-well microplates. For a 96-well plate it takes a total of 27 seconds to switch between wells and then you have to add the actual injection time based on the injection rate entered by the user. Partner with our team of scientists and engineers who can customize your instruments, as well as automate entire workflows to meet the specific needs of your assay, method, or protocol. Mix samples in microplate wells by user-programmable Automix feature. g. An industry-renowned instrument for monitoring GPCRs and ion channels. We would like to show you a description here but the site won’t allow us. For FlexStation I/II/3 plate readers, what are the dead-volumes for the compound plate? The FlexStation plate readers assume a 1 mm pipette height from the bottom of the well when aspirating from a compound source plate. No tools required to assemble. When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. The SpectraMax ABS readers can be integrated with Molecular Devices StakMax® Microplate Stacker within . The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. Contents of wells were pipetted up and down several times to mix. The. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate: 0517 Lit. Next generation SpectraMax® absorbance microplate readers. Devices® SPECTRAmax 190 microplate reader at a wavelength of 600 nm. SpectraMax 250 User. Enter the name of the plate type i. Elevated well walls for easier sealing Round bottom formats Square and round well formats Conform to SBS standards Lids and cap mats available Packed in sleeves of 6, 24. * Note: Programming the washer with a final aspirate height of 4. Narrow bandwidths deliver increased accuracy and enhanced peak resolution. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. The SpectraMax® i3x Multi-Mode Microplate Reader measures spectral-based . Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for. Plates were read in the SpectraMax M5 microplate reader at 485ex/515em with bottom read. Max, FlexStation, Gemini, and earlier instruments as well as import and analysis of data files from FLIPR and Analyst instruments. 5 fg per well to 0. Molecular Spectroscopy Consumables & Accessories Thermal Analysis. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. When a. Reader Documentation Version No. 90349). These models can no longer be repaired. For the second test, the validation kit contained 36 known samples, including 33 DNA samples and 3 no-template controls (NTC). When a. 96-well plate: 0 to 4 Abs, ±2% at 450 nm, 384-well plate: 0 to 3 Abs ±2% at 450 nm: Measurement Type: Endpoint, Kinetic, Spectra, Multipoint, Kinetic Spectra: No. Step 2: Launch SoftMax Pro Software. No additional manuals are available. Absorbance was then determined at 450 nm by SpectraMax Paradigm microplate reader (Molecular. Based on this customer experience and feedback, the Infinite 200 PRO microplate plate reader has been continuously engineered for. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. In the latter case, 8 wells in each column are perfused in series and the 12 columns are. 100 fmol luciferase per well. Endpoint, kinetic, spectrum. Figure 1-2 Match chamfered corners of slide and adapter 2. Molecular Probes 96-well microplates for fluorescence-based assays are black-walled, clear-bottom 96-well microplates that minimize well-to-well crosstalk and. 500 USD. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. Ultra-cooled Photomultiplier Tube and : Silicon photodiode. ©2022 Molecular Devices - All rights. Turn the instrument around so that the back of the instrument is. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. The. The VICTOR Nivo. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and UV-visible absorbance. x. Microplate washers are designed for effortless use with 96- or 384-well plates in routine ELISA applications. Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. The EMax Plus Microplate Reader measures 96-well plates and comes with eight standard filter modes to cover the entire visible range, for applications such as protein quantification, cell viability, cell proliferation and ELISA. #CA961) Methods. 5039228D 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 12 Applications 12 Optics 13 PlateControls 13The microplate reader has been around for more than three decades and continues to serve as a must-have workhorse of clinical, academic research and industrial laboratories alike. Four wash/rinse bottles are included and configurable for both 96- and 384. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. Narrow bandwidths deliver increased measurement accuracy and linearity over the widest range of assays, including. The plate reader can measure multiple readouts, including UV and. 0A-Sunnyvale, CA-Made in USAWe offer a range of multiwell plates including 384-well, 96-well and 24-well plates with a selection of membrane types:. 3. Reconstitution of. Can read 96 well plates (optional 384 well). Component A (2 vials*)Figure 1. 4ti-0255 96 well assay plate, pyramid flat bottom, clear, non-sterile UV wavelength 4ti-0214 UltraVision Plate 384, black, non -sterile, with UV baseELISA Workflow Using the EMax plus Microplate Reader and MultiWash+ Microplate Washer. An example of dual-luciferase assay results. softmax pro software, gemini em reader, molecular devices stakmax microplate stacker, softmax pro data analysis, spectrofluorometer, monochromator microplate reader, cell migration assays, cell. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. Both reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. Fluorescence Microplate Reader Comparison Chart. After incubation, the assay plate is transferred to the seal-peeler to remove the seal from the assay plate and transferred to washing. Our. 000 to 2. You can use 6, 12, 24, 48, 96, and 384-well plates that conform to ANSI/SBS standard microplate footprint and dimensions in the instrument. This page contains the. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. 1. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. Microplate optimization and read height optimization were performed for best results. Click SoftMax Pro Software User Guide. The SpectraMax M4 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96 and 384-well microplates. measurements in compatible Molecular Devices® microplate readers. The list is a standard grouping supplied by Molecular Devices and includes some which cannot be used on this instrument; they can be deleted. 020 fmol 0. No accessories are needed and SoftMax® Pro Software. Microplates, cuvettes (via adapter) Detectors. 00. Molecular Devices SpectraMax Plus 384 Absorbance. x. Microplate optimization and read height optimization were performed for best results. 9000-0756. D. The Phosentix MicroQC™ Plate is an advanced microplate reader analysis tool that offers exceptional multi-modal functionality. Figure 3. Compare specifications and request a quote from leading suppliers. Create custom fluorescence assays without having to purchase expensive filter sets. 384-WELL SBS STERILE MICROPLATE. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. The instrument reads any standard cuvette, microcuvette, test tube and 96 well or UV transparent microplate over full 190-1000 nm wavelength range. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. 20 attomol ATP per well. 000 OD ± 1. PURPOSE. A standard curve consisting of MCF7 cells seeded from 20 to 20,000 per well in a 96-well plate was linear across the entire range with r 2 = 0. Reaction formats The MagMAX™-96 DNA Multi-Sample Kit can accommodate a broad range of throughputs. This can increase throughput and efficiency, thus saving time and space in the lab. Click Add to create the plate. Molecular Devices’ SpectraMax® Plus 384 Microplate Reader provides fast and reliable full spectral range detection for cuvettes, 96-well and 384-well microplates. Gemini EM Reader scans. Quickly image a variety of plate formats and cell types - Image a 96-well plate in under two minutes. Important things to consider when purchasing a multimode microplate reader. Opaque-walled 96- or 384-well tissue culture plates are available as white or black with solid bottoms and white or black with clear bottoms. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. Selects either 96-well or 384-well plate for a stand-alone read. Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. A control well was set up to verify. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. The advanced design of the Sunrise plate reader includes. • Specially formulated optically clear polystyrene and treated to provide protein coating capacity. minutes and begin reading microplates with seven mouse clicks. the use of a reader calibrated with primary NIST and NMI standards. Linear, Orbital Slow, and Orbital Fast. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Learn more. To validate the assays’ ability to detect a wide range of viable cell numbers, a series of cell densities was set up. SpectraMax® Microplate Reader Suite of Products Knowledge Base. Step 4: Add detection antibody – Enzyme-conjugated detection antibody binds to a second site on the. Running both standard spectrophotometer and microplate. Top and bottom reads are. A Costar 24-well tissue culture plate with col1 = blanks and doubling cell a list of plates specifically supported shows, 96, 384, 24, 12, 6 well types. Page 1 of 8 . We would like to show you a description here but the site won’t allow us. Ultraviolet transparent multiwell plates from GreinerThe Accuris SmartReader UV-Vis (model MR9610) is a welcome addition to any lab that is routinely measuring concentration or absorbance in 96 and/or 384 well plates. Some will only support one well density (e. Features. Return to top. Cross-talk <0. 2. QuickSync. HepG2 cells were plated into a 96-well clear-bottom, white-walled plate at 10,000 cells/well and incubated at 37°C overnight. Compare Tool. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs),. e 3370 Costar 96 Well. Materials. View Product. You should ensure that there is enough volume in compound plate wells to operate the IonWorks® system taking into account the dead volume. Resipher 32x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 32x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 32 wells Utilizes Lucid Lab Cloud software for connection to any…. 96-, 384-, and 1536-well microplates. 40266. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). An entire 96-well plate can be processed in less than six seconds. Published Dec 16, 2020 | Updated Jun 22, 2023. (96- or 384-well) simultaneously and with high temporal resolution. x. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. Microplate readers read biological, chemical or physical events that occur on a microtiter plate. Compare this item. One plate received 200 µL of the BCA working reagent, and the other received 200 µL of the Rapid Gold BCA working reagent. OD measurements of bacterial and colloidal bead cultures were performed in a Spectrostar Omega microplate reader (BMG, Germany) with a Costar Flat Bottom 96-well plate with lid and 200 µl per. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Well scans from the Gemini EM Reader illustrating serial dilution of CHO cells using the Live Cell assay from Molecular Probes Live/Dead (Calcein AM/Ethidium Homodimer-1) assay kit. For the name of the nearest authorized Molecular Devices Corporation service facility please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. #SpectraMax L Config) Methods. #C1500) Control standard endotoxin (ACC cat. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. Shaking: Linear, orbital, and double orbital. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. The DynaPro Plate Reader III provides unparalleled levels of productivity and flexibility for the most demanding nanoparticle sizing and protein aggregation tasks such as biotherapeutic formulation, screening of promiscuous inhibitors, and optimization of protein. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. 0% Normal and Rapid modes, 96- and 384-well plates; Absorbance Repeatability: 0. In addition, users can define individual reagents and concentrations to be delivered to each well. #23225). The choice of microplate is a crucial yet often overlooked technical decision in HTS and quantitative biology. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. The Fluorometric Imaging Plate Reader (FLIPR®; Molecular Devices, Sunnyvale, CA) has made a significant contribution to drug discovery programs in the pharmaceutical industry since the first commercial instruments were introduced 9 yr ago. • 0. The clear UltraVision FC 96-well microplate (FC_UltraVision (clear, 96)) showed the best performance of the tested microplates, with absorbance of 0. Top or bottom detection is available with a simple click of a button. The procedures in this protocol support the 96-well reaction plate. May require LS-50B…. Applications include ELISA, protein quantification, enzyme kinetics and cell-based assays. SpectraMax i3x Multi-Mode Microplate Reader. 1 – 2. 5 pM Luminescence performance Wavelength range 300–850 nm Wavelength selection Choice of simultaneous detection of all wavelengths or selection in 1. UV-Star ® 96W Microplate, well working volume 25-370 μL; total well volume 392 μL. as well as all other Molecular Devices' microplate readers. 96 Well Plate Reader found in: Well Plate Reader Accessory, SmartReader™ 96 Microplate Absorbance Reader, SmartReader UV-Vis Microplate Reader, Stratus. VMax . Molecular Devices FMax Thermo Fluoroskan Ascent Fluorescence Microplate Reader W WARRANTY . 100 fmol 384-well (75 µL) 0. This study investigated the efficacy of fermented seaweed (Eucheuma cottonii) on the remediation of fluoride-contaminated soil. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. wells across each plate • Enables viewing and tracking of every well in every plate • Reveals additional information on cellular. The device was able to achieve 3σ limits of detection of ca. The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. Category 2 and 3 microplate readers use gradient heating as well. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. FlexIII. The same volume of genotyping mixture (2x KASP Master mix plus KASP Assay mix) was added to the each well. 6-384 (96- and 384-well with pipetting) 96-well microplates. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set of0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeMolecular Probes® 96-well microplates for fluorescence-based assays are black-walled, clear-bottom 96-well microplates that minimize well-to-well crosstalk and autofluorescence and so are optimized for fluorescence-based applications. 4 (Molecular Devices cat. The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. We would like to show you a description here but the site won’t allow us. Values are averages from 5 clean plates, each filled with 200 µL water/well. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. For measuring the fluorescence or phosphorescence from a 6-, 12-,24-, 48-, or 96-well plate in either the X or Y direction or for luminescence micro densitometry of TLC plates and electrophoresis gels. Shaking: Linear, orbital, and double orbital. FLIPR System with the 540-590 nm BP Emission FLIPR Filter installed (Molecular Devices Corporation) Membrane Potential Assay Kit (Molecular Devices Corporation, R-8034) Clear, flat-bottom, black- or clear-wall 384- or 96-well plates (see consumables list in Appendix A) Clear, polypropylene compound plates (see consumables list in Appendix A) Flexibility to read a variety of plate formats. ~ Bio-Tek ELx808 Microplate Reader $2,795. From the dropdown Menu, select Wellplates. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. This is equivalent to having 511 built-in filters,The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well formats. For the 96-well plate, this equates to 1 fg per well to 1 ng per well; for the 384-well plate it is 0. Capabilities. This is a Molecular Devices Thermomax 96 well Absorbance Microplate Readers. 96- and 384-well microplates. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. Incubation. tools to expand the boundaries of their research. The soil was amended with either 1. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. UT-6550 Microplate Reader is a reliable and robust instrument for a wide. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays.